Fig. 6.

A Wasteosomes from CSF have opsonins on their surface. a wasteosomes purified from CSF and incubated with human plasma become stained with anti-MBL (green). b wasteosomes from CSF and incubated with human plasma become immunostained with anti-C3b (red). c wasteosomes purified from CSF and incubated with PBS instead of human plasma also become immunostained with anti-MBL (green). d wasteosomes purified from CSF and incubated with PBS instead of human plasma also become immunostained with anti-C3b (red). B Hippocampal wasteosomes do not have the opsonins on their surface. a When the hippocampal tissue is double-immunostained with anti-MBL (green) and anti-C3b (red), the wasteosomes do not become immunostained and are observed as a black circle. In this case, one wasteosome can be observed in the center of the image. b When the hippocampal tissue is immunostained with anti-GS and anti-C3b, wasteosomes become stained with anti-GS (green) but not by anti-C3b (red). Scale bars: 25 µm