Figure 3.

(A–C) Example of a control image from the LC used for analysis with labeling of GFAP (green) and DAPI (blue) (A), F-actin (red) and DAPI (blue) (B), and beams of LC (gray) and nuclei (white) by SHG imaging (C). (D–F): Binary analysis used to measure process structures within LC pores (D, E) and beam structure (F). (G–I) Individual higher power images from A to C (red box area) that show greater detail within boxed areas. Arrows (white and red) point to the same LC pore location in each image. Scale bars: 50 µm (A–F) and 20 µm (G–I).