Table 2.
Antibodies and Reagents Used in Serial Cross-Section Studies
| Primary Antibody | Company | Catalog No. | Species | Dilution | Mono- or Polyclonal |
|---|---|---|---|---|---|
| GFAP | Abcam | AB7260* | Rabbit | 1:1000 | Polyclonal |
| GFAP | Sigma-Aldrich | AB5541 | Chicken | 1:500 | Polyclonal |
| IBA1 | Wako | 019-19741 | Rabbit | 1:500 | Polyclonal |
| IBA1 | Abcam | AB5076 | Goat | 1:250 | Polyclonal |
| SOX9 | Sigma-Aldrich | AB5535 | Rabbit | 1:500 | Polyclonal |
| TMEM119 | Cell Signaling Technology | 90840 | Rabbit | 1: 250 | Monoclonal |
| Collagen IV | SouthernBiotech | 1340-01 | Goat | 1:500 | Polyclonal |
| Myelin basic protein | Abcam | AB7349 | Rat | 1:1000 | Monoclonal |
| Additional Labels | Company | Catalog No. | Description | Dilution | Excitation Wavelength (nm) |
| Phalloidin 568 | Invitrogen | A12380* | F-actin label | 1:70 | |
| DAPI 405 | Roche | 10-236-276-001* | Nuclear stain | 1:1000 | |
| Secondary Antibody | Company | Catalog No. | Species | Dilution | Excitation Wavelength (nm) |
| Alexa Fluor 488 | Invitrogen | A11008 | Goat anti-rabbit | 1:500 | 488 |
| Alexa Fluor 488 | Invitrogen | A21206 | Donkey anti-rabbit | 1:500 | 488 |
| Alexa Fluor 488 | Invitrogen | A11055 | Donkey anti-goat | 1:500 | 488 |
| Alexa Fluor 568 | Invitrogen | A11011 | Goat anti-rabbit | 1:500 | 561 |
| Alexa Fluor 568 | Invitrogen | A10042 | Donkey anti-rabbit | 1:500 | 561 |
| Cy3 | Jackson ImmunoResearch | 703-165-155 | Donkey anti-chicken | 1:500 | 561 |
| Alexa Fluor 647 | Invitrogen | A21447 | Donkey anti-goat | 1:500 | 633 |
| Alexa Fluor 647 | Invitrogen | A21247 | Goat anti-rat | 1:500 | 633 |
| Blocking Sera | Company | Catalog No. | Species | Concentration | |
| Normal goat serum | Jackson ImmunoResearch | 005-000-121 | Goat | 0.5% | |
| Normal donkey serum | Jackson ImmunoResearch | 017-000-001 | Donkey | 0.5% | |
*
These labels were used for the quantification portion of the study.