Table 3.

Imaging Parameters Used for Quantitative Study

Parameters	Confocal Roadmap	Quantitative Confocal	Quantitative SHG
Objective used	Plan-Apochromat, 20×/0.8 dry	Plan-Apochromat, 40×/1.3 oil	Plan-Apochromat, 40×/1.3 oil
Markers imaged	GFAP/phalloidin/DAPI	GFAP/phalloidin/DAPI	SHG for collagen beams
Number of tiles	7 × 7 = 49	2 × 2 = 4	2 × 2 = 4
Stitched image resolution, pixels	6430 × 6430	1924 × 1924	1924 × 1924
Stitched image size	2.67 × 2.67 mm	399.36 × 399.36 µm	399.36 × 399.36 µm
Stitching overlap, %	12	0	0
Lasers used, nm	405, 488, 561	405, 488, 561	2 photon at 790
Detection wavelengths, nm	410–495, 495–572, 574–712	410–495, 495–572, 574–712	390–410
Averaging used	1	2	1
Pixel dwell time, µs	0.79	1.58	6.3
Slides stained per eye, n	3–5	3–5	3–5
Images peripheral (LC/eye), n	Not applicable	10–12	10–12
Images central (LC/eye), n	Not applicable	10–12	10–12
Total images analyzed per eye, n	Not applicable	20–24	20–24