Table 4.
Quantified Parameters of ONH Components
| Tissue Component | Structural Parameter |
|---|---|
| GFAP in pores | Area fraction |
| Mean ± SD of process width | |
| Maximum process width | |
| GFAP overall | Area fraction |
| Mean ± SD of process width | |
| Maximum process width | |
| F-actin in pores | Area fraction |
| Mean ± SD of process width | |
| Maximum process width | |
| F-actin overall | Area fraction (F-actin area/mask area) |
| Mean ± SD of process width | |
| Maximum process width | |
| Beam structure (SHG) | Area fraction |
| Mean ± SD of beam width | |
| Maximum beam width | |
| Pore area (negative SHG space) | Mean ± SD area of pores |
| Nuclei overall | Total nuclear area |
| Median of nuclear area | |
| Mean ± SD of nuclear area | |
| Median aspect ratio of nuclei | |
| Mean ± SD of aspect ratio of nuclei | |
| Count of nuclei | |
| Percentage of nuclei overall | |
| Area percentage of nuclei overall | |
| Nuclei in pores | Total nuclear area |
| Median of nuclear area | |
| Mean ± SD of nuclear area | |
| Median aspect ratio of nuclei | |
| Mean ± SD of aspect ratio of nuclei | |
| Count of nuclei | |
| Count/area | |
| Percentage of nuclei in pores | |
| Area percentage of nuclei in pores | |
| Nuclei on beams | Total nuclear area |
| Median of nuclear area | |
| Mean ± SD of nuclear area | |
| Median aspect ratio of nuclei | |
| Mean ± SD of aspect ratio of nuclei | |
| Count of nuclei | |
| Count/area | |
| Percentage of nuclei on beams | |
| Area percentage of nuclei on beams | |
| Axonal compartments | Number of axonal compartments |
| Total axonal area | |
| Median of axonal area | |
| Mean ± SD of axonal area |
Linear measures were expressed in units of µm and areas in µm2.