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. 2022 Oct 29;12:18259. doi: 10.1038/s41598-022-23187-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Germ layer marker expression detected by ISH during gastrulation at 5 hpf (A) and 7 hpf (B). Orientation: ntla and sox17, dorsal views with animal pole to the top at 75% epiboly stage; dorsal-animal views with anterior towards the top: otx2; others, animal-pole view (eve1, chd, gsc and gata2a) with dorsal to the right. The scatter plot on the right corresponds to the statistical results of hybridization signals. Scale bars, 100 μM.