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. 2022 Oct 29;12:18259. doi: 10.1038/s41598-022-23187-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Marker expression at 24 hpf. Lateral views with anterior to the left. (B) Heart morphology (marked by the pan-cardiomyocyte marker myl7) at 48 hpf. Embryo orientation: ventral view with the anterior at the top. Arrows point to the atrium; red lines demonstrate the acute angle formed between the atrial and ventricular axes. The scatter plot on the right corresponds to the statistical results of angles.