Table 2.
Primers Used for RT-qPCR
| Gene | Primer Sequence |
|---|---|
| GAPDH | F: 5’-CCCTTAAGAGGGATGCTGCC-3’ |
| R: 5’-TACGGCCAAATCCGTTCACA-3’ | |
| TNF-α | F: 5’-CTGAACTTCGGGGTGATCGG-3’ |
| R: 5’-GGCTTGTCACTCGAATTTTGAGA-3’ | |
| iNOS | F: 5’-TGGTGAAGGGACTGAGCTGT-3’ |
| R: 5’-CTGAGAACAGCACAAGGGGT-3’ | |
| IL-1β | F: 5’-TGGAGAGTGTGGATCCCAAG-3’ |
| R: 5’-GGTGCTGATGTACCAGTTGG-3’ | |
| Arg-1 | F: 5’-CACCTGAGTTTTGATGTTGATGG-3’ |
| R: 5’-TCCTGAAAGTAGCCCTGTCTTGT-3’ | |
| CD206 | F: 5’-ACGAGCAGGTGCAGTTTACA-3’ |
| R: 5’-ACATCCCATAAGCCACCTGC-3’ | |
| IL-10 | F: 5’-GAGAAGCATGGCCCAGAAATC-3’ |
| R: 5’-GAGAAATCGATGACAGCGCC-3’ |