Table 2.
Performance of Manual and Automated Dual-Stain Cytology for Detection of Anal Precancer Among 320 Human Immunodeficiency Virus–Positive Men Who Have Sex With Mena
| Variable | DS Result, No./Total No. (%) | |
|---|---|---|
| Manual DS | Automated DS | |
| Threshold | 1 Cell positive | 3 Cells positive |
| Positivity | 215/320 (67.2)a | 196/320 (61.3)a |
| Detection of AIN2+ | ||
| Sensitivity | 81/88 (92.1) | 82/88 (93.2) |
| Specificity | 98/232 (42.2)a | 118/232 (50.9)a |
| PPV | 81/215 (37.7)b | 82/196 (41.8)b |
| NPV | 98/105 (93.3) | 118/124 (95.2) |
| Detection of AIN3 | ||
| Sensitivity | 47/50 (94.0) | 48/50 (96.0) |
| Specificity | 102/270 (37.8)a | 122/270 (45.2)a |
| PPV | 47/215 (21.9)b | 48/196 (24.5)b |
| NPV | 102/105 (97.1) | 122/124 (98.4) |
Abbreviations: AIN2+, anal intraepithelial neoplasia (AIN) grade 2 or 3 by cytology and/or histology; AIN3, AIN grade 3 by cytology and/or histology; DS, dual-stain cytology; NPV, negative predictive value; PPV, positive predictive value.
Significant at P < .05 for comparison between automated and manual DS (McNemar χ2 test).
Significant at P < .05; predictive values were evaluated with the R package DTComPair, using the generalized score statistic.