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. Author manuscript; available in PMC: 2023 Jul 7.
Published in final edited form as: Cell. 2022 Jun 22;185(14):2523–2541.e30. doi: 10.1016/j.cell.2022.05.024

Figure 1: Efficient generation of human primitive streak and lateral mesoderm from hPSCs within 1 and 2 days, respectively.

Figure 1:

A) Artery and vein development

B) Generating MIXL1+ mid primitive streak within 24 hours of hPSC differentiation (Loh et al., 2016), assayed by flow cytometry of MIXL1-GFP hPSCs (Davis et al., 2008)

C) VEGF and BMP specify, whereas TGFβ and WNT repress, day 2 lateral mesoderm. Day 1 hPSC-derived primitive streak was treated with the indicated signals (concentrations indicated, and 10 nM Axitinib; 200 ng/mL NOGGIN; 1 μM DMH1, XAV939 and C59; 2 μM SB505124 and SB431542; base conditions contained 10 ng/mL BMP4 and/or 100 ng/mL VEGF) for 24 hours; qPCR was performed on day 2.

See also Figure S1 and Table S5