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. Author manuscript; available in PMC: 2023 Jul 7.
Published in final edited form as: Cell. 2022 Jun 22;185(14):2523–2541.e30. doi: 10.1016/j.cell.2022.05.024

Figure 5: Nipah and Hendra viruses target artery cells.

Figure 5:

A) Syncytia (arrows) in H1 hPSC-derived artery and vein ECs inoculated with Nipah or Hendra viruses for 24 hours; scale = 25 μm

B) Numbers of nuclei per cell after inoculation with Nipah or Hendra viruses for 24 hours (top); syncytia frequency (≥3 nuclei/cell; bottom)

C) Live imaging of cocultured SUN004.1.9 GFP+ and H1 wild-type artery ECs infected with Nipah virus, tracking cell fusion into GFP+ syncytia, followed by death (scale = 200 μm, timestamp: hours:minutes)

D) Live imaging showed Nipah- and Hendra-infected artery ECs displayed significantly greater median velocity (a measure of cell fusion) than vein ECs

E) Fusion and GFP diffusion between 3 Nipah-infected artery ECs, followed by aggregation of their nuclei (scale bar = 20 μm, timestamp: hours:minutes)

See also Figure S5 and Movie S1