Figure 6: Artery cells express higher levels of EFNB2, the Nipah and Hendra virus receptor.
A) Nipah and Hendra viral RNA replication in H1 hPSC-derived artery and vein ECs, as quantified by qPCR of culture media; *P<0.05; **P<0.01
B) scRNAseq of human adult lung ECs (Travaglini et al., 2020)
C) Higher EFNB2 levels in hPSC-derived artery ECs relative to vein ECs, as shown by i) scRNAseq of day 3 artery vs. day 4 vein ECs and ii) flow cytometry for surface EFNB2 protein expression on artery vs. vein ECs, 4 days post-expansion
D) Nipah or Hendra viral RNA replication in H1 and EFNB2−/− hPSC-derived artery and vein ECs, as assayed by qPCR of culture media
E) H1 and EFNB2−/− hPSC-derived artery and vein ECs inoculated with GFP-encoding Nipah pseudovirus (Palomares et al., 2013); **P<0.01