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. 2022 Oct 29;13:6482. doi: 10.1038/s41467-022-33957-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, b Orthogonal slices of original (a) and IsoNet-processed (b) tomograms of isolated ribosomes. c 3D rendering of the missing-wedge corrected tomogram. Dashed lines show the air-water or carbon-water interfaces. d Enlarged view of the tomogram showing the structures of individual ribosome proteins. e Densities of ribosomes in IsoNet-processed tomograms (transparent surface) fitted with atomic models of the ribosome. Green ribbons: large ribosomal subunits; Orange ribbons: small ribosomal subunits.