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. 2022 Oct 29;13:6482. doi: 10.1038/s41467-022-33957-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b Orthogonal slices of a tomogram of flagellar for SIRT reconstruction (a) and IsoNet reconstruction (b). DMT doublet microtubule, CPC central-pair complex, RS radial spoke, Red arrows outer arm dyneins, Blue arrow inner arm dynein. c YZ slices show the cross-sections corresponding to the cyan lines in (a) and (b). d 3D rendering of PFR in the IsoNet-generated tomogram. The left panel is the 3D view of PFR in the direction corresponding to (c). The right panel shows the en face view of a PFR scissors-stack-network plane.