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. 2022 Oct 29;13:6482. doi: 10.1038/s41467-022-33957-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, b Orthogonal slices of a synaptic tomogram reconstructed with WBP (a) and IsoNet (b). SV: synaptic vesicle; Mito mitochondria, MT microtubule, PSD postsynaptic density. c, d Zoomed-in orthogonal slices of WBP reconstruction and IsoNet-produced reconstruction. Magenta arrows: vesicle linker; Orange arrows: small cellular proteins; Green arrows: microtubule luminal particles; Red arrows: microtubule subunits. e 3D rendering of the tomogram shown in (b). f 3D rendering of a slab of tomogram with WBP reconstructions and Isotropic reconstructions, showing microtubules and vesicles.