Figure 7.

DE does not alter the strength of A1 L6 feedback excitatory synaptic transmission to MGBv. A, Circuit diagram highlighting A1 L6 inputs to MGBv. B, Confocal tiled image of a coronal section showing ChR2-YFP-expressing A1 L6 neurons and axonal labeling in MGBv (green). The section was counterstained with DAPI (blue). C, ChR2-evoked Sr²⁺-mEPSCs recorded from MGBv neurons. Left, Example traces from NR and DE mice. A 5 ms LED pulse duration was used. Labels are the same as in Figure 1D. Middle, Comparison of the average amplitude of ChR2-evoked Sr²⁺-mEPSCs (NR: 12.3 ± 0.4 pA, 16 cells from 8 mice; DE: 11.5 ± 0.5 pA, 12 cells from 5 mice; t test: t₍₂₆₎ = 1.267, p = 0.2165). Bars, Mean ± SEM; gray circles, average value for each cell. Right, Average ChR2-evoked Sr²⁺-mEPSC traces from NR and DE.