Figure 6.

Activity-induced nascent proteomic dynamics in excitatory cortical neurons following 7 d of ANL treatment. A, Schematic of experimental protocol. Adult mMetRS^KI/WT/EMX^cre mice were treated with a daily dose of ANL (830 mg/kg, i.p.) for 1 week followed by a single dose of PTZ (30–39 µg/kg, i.p.) to induce seizure. The brain was harvested 18–20 h later. Cortical tissue was processed to tag ANL-containing proteins with heavy or light isotopically labeled biotin-alkyne using click chemistry before LC-MS/MS analysis of biotinylated peptides. B, Volcano plot showing quantitative proteomic analysis of PTZ versus control samples. Statistically significantly (p < 0.05, |FC|>1.5) increases or decreases are labeled in red or blue, respectively. N = 4 pairs of control and PTZ-treated mice, with 3 pairs of males and 1 pair of females (Extended Data Fig. 6-1, see for list of all quantified peptides). C, D, Protein classes (C) and cellular localization (D) of the NSPs labeled with ANL [Extended Data Figs. 6-2 and 6-3, see for data on Top Protein Categories (Panther) and Protein Subcellular Localization (IPA)].