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. Author manuscript; available in PMC: 2022 Oct 29.
Published in final edited form as: Cell Rep. 2022 Sep 27;40(13):111431. doi: 10.1016/j.celrep.2022.111431

Figure 3. NMDA receptor activation declusters DAT in mouse brain slices.

Figure 3.

(A) Workflow diagram.

(B) Data processing steps to isolate single varicosities from dSTORM images.

(C) Representative DAT dSTORM image before, during, and after varicosity identification.

(D) Example varicosity from control, NMDA-, and AP5 plus NMDA-treated slices (clusters with diameter >75 nm in green, clusters < 75 nm in magenta). Right: normalized fraction of DAT clusters greater than 75 nm (percent). Means ± S.E. from 3 experiments with analysis of 1,793 control, 1,102 NMDA, and 1,033 NMDA plus AP5 varicosities; one-way ANOVA, ****p < 0.0001, *p < 0.05.

(E) Example varicosity from control, NMDA (20 μM)-, and AP5 plus NMDA (20 μM)-treated slices (dense clusters in red, >80 localizations; radius, 50 nm). Right: normalized fraction of DAT localizations in dense clusters (percent). Means ± S.E.; one-way ANOVA, ****p < 0.0001, ***p < 0.001, **p < 0.01.

(F) The PDFs for the Voronoi tessellated areas, averaged by varicosity.