| Antibodies |
|
| Rat Anti-DAT |
Millipore |
MAB369 RRID:AB_2190413 |
| Mouse Anti-TH |
Millipore |
MAB318 RRID:AB_2201528 |
| Rabbit Anti-tRFP |
Evrogen |
AB233 RRID:AB_2571743 |
| Rabbit Anti-D2R |
Synaptic Systems |
376 203 RRID:AB_2636918 |
| Mouse Anti-STX1 |
Synaptic Systems |
110 011 RRID:AB_887844 |
| Mouse Anti-PIP2 |
Abcam |
Ab11039 RRID:AB_442848 |
| Mouse Anti-Clathrin light chain |
Synaptic Systems |
113 011 RRID:AB_887706 |
| Donkey Anti-Rat |
Jackson |
712-005-153 RRID:AB_2340631 |
| Donkey Anti-Mouse |
Jackson |
715-005-151 RRID:AB_2340759 |
| Donkey Anti-Rabbit |
Jackson |
711-005-152 RRID:AB_2340585 |
| Mouse Anti-synaptotagmin 1 |
Synaptic systems |
105 311 RRID:AB_10805653 |
|
| Bacterial and virus strains |
|
| pAAV-CAG-Flex-NES-jRGECO1a-WPREpA |
Addgene |
RRID: Addgene #100854 |
| pAAV-pTH-iCre-WPREpA |
This paper |
N/A |
| pAAV-hSyn-DIO-mNaChBac-T2A-mKate2-WPREpA |
This paper |
Adapted from RRID: Addgene #60658 |
| pAAV-hSyn-DIO-mNaChBac-MUT- T2A-mKate2-WPREpA |
This paper |
Adapted from RRID: Addgene #60658 |
| pAAV-hSyn-DIO-Kir2.1-T2A-mKate2-WPREpA |
This paper |
Adapted from RRID: Addgene #60661 |
|
| Chemicals, peptides, and recombinant proteins |
|
| Alexa647 NHS ester |
ThermoFisher |
A20006 |
| CF568 NHS ester |
Biotum |
92131 |
| NMDA (N-methyl-D-aspartate) |
Sigma |
M3262 |
| DL-AP5 |
Tocris |
0105 |
| omega-conotoxin GVIA |
Abcam |
Ab120215 |
| omega-agatoxin IVA |
Abcam |
ab120210 |
| Quinpirole |
Sigma-Aldrich |
Q102 |
| Haloperidol |
Sigma-Aldrich |
H1512 |
| Nomifensine |
Tocris Bioscience |
1992 |
| Cocaine |
Sigma-Aldrich |
C5776 |
| JHW007 |
Synthesized by Amy Newman |
N/A |
| Noribogaine |
Gift from Sacrament of Transition (Maribor, Slovenia) |
N/A |
| Dopamine |
Sigma-Aldrich |
H8502 |
| UH-232 |
Tocris |
0775 |
| DG3-63 |
Guthrie et al., 2020
|
N/A |
| DG3-80 |
Guthrie et al., 2020
|
N/A |
| NeuroMag reagent |
OZ biosciences |
NM50200 |
|
| Experimental models: Cell lines |
|
| Cath.a-differentiated (CAD) cells (Qi et al., 1997) |
Sigma |
08100805 |
| HEK cells (human embryonic kidney cells) |
ATCC |
ATCC CRL-1573 |
| Rat primary midbrain dopaminergic cultures from pups of Wistar IGS rats |
This paper |
N/A |
|
| Experimental models: Organisms/strains |
|
| Timed pregnancy, day 15, Wistar IGS rats |
Scanbur (Charles River) |
94-003-WI-F-TP15 |
| C57BL/6J mice |
Scanbur (Charles River) |
000664 |
|
| Recombinant DNA |
|
| pmEOS2-hDAT |
This paper |
Generated from pmEOS2-C1 (RRID: Addgene #54510) |
| pRC/CMV hDAT |
Herborg et al., 2018
|
N/A |
| pRC/CMV hDAT D421N |
Herborg et al., 2018
|
N/A |
| pcDNA 3.1-SF-D2s |
Klewe et al., 2008
|
N/A |
|
| Software and algorithms |
|
| ThunderStorm |
Ovesny et al., 2014
|
N/A |
| Redundant cross-correlation |
Wang et al., 2014
|
N/A |
| DBSCAN |
This paper |
https://doi.org/10.5281/zenodo.7016376
|
| Voronoi Tessellation |
This paper |
https://doi.org/10.5281/zenodo.7016376
|
| Varicosity segmentation |
This paper |
https://doi.org/10.5281/zenodo.7016376
|
|
Swift
|
Ulrike Endesfelder Lab, Bonn University |
https://swifttracking.slack.com/join/shared_invite/zt-m6e3fb9u-J2lBX3uaHY0v3JZwiOeSw#/shared-invite/email
|
| dSTORM colocalization |
This paper |
https://doi.org/10.5281/zenodo.7016376
|
