Figure 5.

The NFKB1 gene DD mutant caused aberrant mitochondria fission. All cells were stained with MitoTracker Green FM and then analyzed by ImageJ. (a) Representative confocal photomicrographs showing mitochondrial morphology imaged by laser scanning confocal microscopy. (b) The percentage of cells with fragmented or spherical mitochondria in each group. HUVECs were treated with 25 mM glucose and 250 μM palmitic acid (PA) for 3 hours. Data represents the mean ± SEM of at least three independent cell culture preparations (more than 10 visual fields per group). ^∗P < 0.05.