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. Author manuscript; available in PMC: 2023 Oct 27.
Published in final edited form as: Cell. 2022 Oct 14;185(22):4170–4189.e20. doi: 10.1016/j.cell.2022.09.008

Figure 3. TRPV1+ nociceptor ablation leads to a transmissible dysbiosis.

Figure 3.

(A) Fecal microbial composition was analyzed by 16S rRNA gene sequencing with principal coordinates analysis in DMSO- or RTX-treated mice. (B) Averaged relative abundance at the phylum level. (C) Phylum-specific qPCR of fecal samples from DMSO- or RTX-treated mice. (D) FMT donor and recipient fecal microbial composition was analyzed by 16S rRNA gene sequencing, presented as averaged relative abundance at the genus level. (E to H) Disease and recovery of DSS-treated FMT mice were monitored by daily weight loss (E), clinical disease score (F), colon length (G) and H&E staining of distal colon (H) at day 10. (I, J) Frequency of colonic neutrophils from mice in (E) on day 10, pre-gated on live, CD45+ events. (K to N) Disease and recovery of DSS-treated cohoused mice were monitored by daily weight loss (K), clinical disease score (L), colon length (M), and H&E staining of distal colon (N) at day 10. Scale bars = 50 μm in (H) and (N). Data are representative of two independent experiments with n=3–5 per group. Data are Mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.