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. 2022 Oct 29;8:433. doi: 10.1038/s41420-022-01225-9

Fig. 3. BAU-243 induced limited apoptosis and cell cycle arrest in low Bcl-2 expressing cells.

Fig. 3

a Flow cytometric apoptosis analysis of A172 cells by Annexin V/PI staining, treated with DMSO (0.1%), ABT-199 (10 µM), and BAU-243 (20 µM) for 72 h. Annexin V is detected on the APC channel, and PI is detected on the PE channel. The bottom left quadrant shows viable cells, the bottom right quadrant shows early apoptotic cells, the top right quadrant shows late apoptotic cells, and the top left quadrant shows necrotic/dead cells. b Western blots of apoptosis-related proteins PARP, cleaved-PARP, pro-caspase-9, and pro-caspase-3. Total protein was isolated from A172 cells. Cells were either treated with 1 µM of staurosporine for 4 h, DMSO, ABT-199, or BAU-243 for 72 h. 50 µg per lane was loaded. GAPDH was used as an internal control. c Cell cycle analysis of A172 cells with PI staining, treated with DMSO (0.1%), ABT-199 (10 µM), and BAU-243 (20 µM) for 72 h. PI is detected on the PE channel. d Cell cycle stage fractions of A172 cells, showing G1, S, and G2/M phases.