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. 2022 Oct 29;12:18217. doi: 10.1038/s41598-022-22969-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Production of p-nitroaniline by monitoring absorption intensity at 405 nm after UV irradiation to aq.solutions of chymotrypsin. UV doses in mJ/cm² are (Blue diamond) 100 and (Blue circle) 500 at 222 nm, (green diamond) 100 and (green circle) 500 at 254 nm, (Black circle) 0. The initial hump is caused by light scattering. n = 1.