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. 2022 Oct 29;5:1149. doi: 10.1038/s42003-022-04150-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Immunofluorescence at 40× (scale bar of 50 µm) of COPD human bronchial epithelial cells differentiated at week one to three of air–liquid interface (ALI) show decreased expression of E-cadherin and β-tubulin (ciliated cells marker) expression, increase expression of MUC5AC (goblet cell marker), without any changes in Cytokeratin 14 (Basal cell marker), as compared to non-diseased human bronchial epithelial (normal) cells.