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. 2022 Oct 17;119(43):e2211065119. doi: 10.1073/pnas.2211065119

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Copyright © 2022 the Author(s). Published by PNAS.

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Fig. 1. — VHH21 binds to Ly6G/C-positive cells in an influenza virus–infected lung. (A) Schematic of site-specific conjugation of VHH21 with biotin-azide using sortase, followed by a PEGylation step. (B) The installation of PEG20 affects binding of SDS to the modified protein and is responsible for its heterogenous migration on SDS-PAGE. (C) Schematic showing the experimental outline. (D) Immunohistochemistry of uninfected and virus-infected lung sections at 10× and 20× magnification. IAV, influenza A virus.