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Characterization of 89Zr-labeled VHHs. (A) Schematic representation of 89Zr-labeling and PEGylation of VHHs. (B) LC-MS and SDS-PAGE show the purified and modified versions of the indicated VHHs. The installation of PEG20 affects binding of SDS to the modified protein and is responsible for its heterogenous migration on SDS-PAGE. DFO, deferoxamine; IAV, influenza A virus.
