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. 2022 Oct 17;119(43):e2123476119. doi: 10.1073/pnas.2123476119

Fig. 3.

Fig. 3.

Marker gene expression and transcriptome of MGLs compared with stem cells and other myeloid cell types. (A) Real-time qPCR showing gene-expression levels of the pluripotency factors, the MAC TF MYB, core microglia network TFs, core microglia signature genes, and typical microglia cell-surface proteins in MGLs. (B) Comparison of expression levels of microglia signature genes and MYB in MGLs versus peripheral blood–derived MACs (serum-free), monocytes (Monos), and dendritic cells (DCs). Statistical significance was determined using Bonferroni-corrected one-way ANOVA (*P < 0.05; **P < 0.01; ***P < 0.001). (C) Multidimensional scaling plot of bulk RNA sequencing data of our MGLs generated with different media conditions, human postmortem microglia ex vivo, PBMC-isolated monocytes, and PBMC-derived MACs with different media conditions. Moreover, the plot contains RNA-expression profiles of MGLs from three previously published protocols (9, 19, 25), including additional cell types for comparison, and an extensive dataset of primary human microglia in vitro and ex vivo, MACs, and monocytes (8).