Fig. 4.

MGLs exert classical myeloid and microglia-specific functions. (A) ROS production of MGLs and MACs at baseline and responding to treatment with the NADPH-oxidase activator PMA under standard conditions and after treatment with the selective NOX2 inhibitor GP91-TAT. (B) Differentially secreted cytokines in MGLs, MACs, and monocytes in NGD medium (MonoNGD) stimulated with IFN-γ and LPS. (C) Representative trace of Ca²⁺ oscillations induced by sequential stimulation of MGLs with ADP, ATP, and ionomycin (Left). Upon stimulation with ADP, MGLs respond with an increased fluorescence, indicating Ca²⁺ influx, and a subsequent return to baseline, indicating Ca²⁺ efflux (Middle). Quantification of calcium transients in MGLs and MACs evoked by ATP or ADP stimulation (Right). Statistical significance was determined using Bonferroni-corrected one-way ANOVA (ns: not significant; *P < 0.05; **P < 0.01; ***P < 0.001).