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. 2022 Oct 17;119(43):e2205350119. doi: 10.1073/pnas.2205350119

Fig. 1.

Fig. 1.

COP1 inhibits GATA2 and AR in PCa cells. (A) COP1 expression in human PCa cell lines. (B) Knockdown of COP1 in LNCaP and 22Rv1 cells using three different COP1 siRNAs (siCOP1, 1–3). Control (Ctrl), siRNA targeting luciferase. (C) Knockdown of COP1 in LNCaP and LAPC4 cells using two independent shRNAs (shCOP1, 1–2). NT, nontargeting control. The engineered (D) LAPC4, LNCaP, (E) 22Rv1, and (F) C4-2B cells with Dox-inducible expression of Flag-tagged COP1 (iCOP1) or control (iCtrl) were treated with 0.5 µg/mL Dox for 5 d before Western blotting analysis. All data shown in A–F are Western blots. (G) qPCR analysis of gene expression of engineered LNCaP, LAPC4, C4-2B, and 22Rv1 iCOP1 cells versus iCtrl cells induced with 0.5 µg/mL Dox for 5 d. Quantification data as means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. ns, not significant. Data are representative of at least three independent experiments.