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. 2022 Oct 17;119(43):e2210912119. doi: 10.1073/pnas.2210912119

Fig. 4.

Fig. 4.

Sublethal concentrations of PS757 increase influx and decrease efflux in OG1RF. (A) EtBr accumulation in OG1RF cells treated with vehicle (DMSO, green) or PS757 (pink). EtBr accumulation results are plotted as fold-change of EtBr fluorescence over a 30-min time course. (B) Relative fluorescence of EtBr depletion of OG1RF cells pretreated with EtBr and i) vehicle (DMSO, green); or ii) PS757 (pink). (C) Relative fluorescence of PS757 depletion of OG1RF cells pretreated with EtBr and i) vehicle (DMSO, green); or ii) PS757 (pink). EtBr and PS757 depletion results are plotted as % relative EtBr fluorescence (respective to the initial fluorescence, 100% at t = 0) over a 30-min time course. Cells were pretreated with EtBr (1 µg/mL), DMSO (0.05%) and/or PS757 (5 µM). The fluorescence signals emitted from PS757 (λEx = 400 nm and λEm = 440 nm) and EtBr (λEx = 544 nm and λEm = 590 nm) were measured with SpectraMax iD3 microplate reader (Molecular Devices). All measurements were performed in triplicate and results were plotted using GraphPad Prism (version 9.3.1). Black bars represent SDs of replicates.