Skip to main content
. 2022 Feb 26;31(15):2521–2534. doi: 10.1093/hmg/ddac021

Figure 4.

Figure 4

eIF2A, eIF2D and DENR are not required for stress-induced RAN translation. (A–C) HEK293 cells were transfected with eIF2A, eIF2D or DENR siRNAs, respectively, as well as a non-targeting control siRNA for 24 h before transfection with NLuc reporter plasmids. 19 h post reporter transfection, HEK293 cells were treated with 2 μM thapsigargin for 5 h, and then NLuc levels were measured. NLuc levels are expressed relative to vehicle (DMSO) treated cells. (D–E) NLuc expression for indicated reporter mRNAs expressed in RRL treated with 10 μM NSC119893, expressed relative to vehicle treated controls, n = 6. All graphs represent mean with error bars ± standard deviation. (A–C) Two-way analysis of variance (ANOVA) with Sidak’s multiple comparison test. (D–E) One-way ANOVA with Dunnett’s multiple comparison test, *P < 0.05, **P < 0.01, ****P < 0.0001.