Table 3.
Primary antibody information
| Antibody target | Product information | Species | Probing conditions |
|---|---|---|---|
| eIF2A | Protein Tech, 11 233-1-AP | Rabbit | 1:8000 – 1 h at room temperature |
| eIF2A | Abcam; ab169528 | Rabbit | 1:1000 – overnight at 4°C |
| eIF2D | Protein Tech, 12 840–1-AP | Rabbit | 1:1000 – overnight at 4°C |
| DENR | Sigma, Clone 1H3, WH0008562M1 | Mouse | 1:1000 – overnight at 4°C |
| MCTS1 | Sigma, SAB2701331 (discontinued) | Rabbit | 1:500 – overnight at 4°C |
| FLAG | Sigma, M2, F1804 | Mouse | 1:1000 – overnight at 4°C |
| GAPDH | SCBT, sc-32 233 | Mouse | 1:1000–1 h at room temperature |