Figure 2.

Proteomics identifies dysregulation of Electron Transport Chain complex I in the rd1 retina before degeneration onset. (A) Venn diagram comparison of differentially expressed proteins in P6 and P10 rd1 retina. (B) Summary of pathway enrichment analysis that identifies differential regulation at early stages (P6) in the rd1 retina. Ridge plots show fold change (rd1 versus WT) distribution of leading-edge proteins from significant genesets. The top and bottom panels show over- and under-enriched pathways in red and blue colors respectively. See also Supplementary Material, Figure S3B for late stage (P10) data. (C) Proteomic fold change (FC) of mitochondrial electron transport chain complexes. Black dots inside violin plots represent significant differential expression of an individual protein (rd1 versus WT) of a specific complex. Gray dots inside violin plots represent non-significant differential expression. (D) Dumb bell plot showing change in protein expression from P6 to P10 for leading-edge proteins of oxidative phosphorylation. Dark red and purple dots represent significantly different proteins at P6 and P10, respectively. Grayed dots represent proteins with expression less than statistical significance threshold. See also Supplementary Material, Figure S3C.