Skip to main content
. Author manuscript; available in PMC: 2022 Oct 30.
Published in final edited form as: Sci Transl Med. 2022 Mar 16;14(636):eabl9945. doi: 10.1126/scitranslmed.abl9945

Fig. 3. CDMCs and microglia are transcriptionally distinct cells.

Fig. 3

(A) Principal component analysis (PCA) plot for microglia (n=4, green), CDMC (BMT+P) (n=3, purple), and CDMC (BMT only) (n=2, yellow) using 2,500 most variable genes. Ellipses demarcate 95% confidence interval for assigned clusters.

(B) Unsupervised hierarchical clustering of microglia, CDMC (BMT+P) and CDMC (BMT only).

(C) Unsupervised hierarchical clustering of our dataset and a published dataset of various microglia-like cells of different origins (24). Our samples are shown as open triangles and the published data as filled circles. CDMC after BMT with PLX5622 treatment (CDMC (BMT+P)), CDMC after conventional BMT without PLX5622 treatment (CDMC (BMT only)), microglia (MG), intracranial transplanted (ICT)-P5 microglia (ICT P5 MG), ICT-cultured microglia (ICT cultured MG), ICT-adult microglia (ICT MG), ICT- yolk sac cells (ICT YS), ICT-fetal brain cells FB (ICT FB), ICT-fetal liver cells (ICT FL), ICT-BM cells (ICT BM), ICT-PB (ICT Blood), Bone marrow transplanted by IP (BMT).

(D) Quantitative real-time PCR analysis of bone marrow-derived microglia-like cell marker Ms4a7 and Clec12a in microglia and CDMCs (Mean ± SEM, n=6, *p < 0.05, **p < 0.01, ns: not significant, ANOVA).

(E) Overlaid density plots of CDMCs (red) of BMT+PLX5622 protocol (BMT+P) at the indicated time points and myeloid cells of untransplanted mice (gray).

(F) Representative images of GFP+ and TMEM119+ cells in the cortex on D83. Scale bars: 50 μm.