TABLE 2.

Cultivation data and calculated parameters for fed‐batch high cell density cultivation of C. glutamicum MB001(DE3) pMKEx2‐ eyfp using acetate as carbon source. Values and error bars represent means ± standard deviations from biological duplicate cultivations.

Cultivation parameter	Values
Process time [h]	27
cbiomass max [gCDW/L]	67.8 ± 1.9
Reactor volume [L]	15.9 ± 1.3
mbiomass max produced [gCDW]	1074 ± 115
Acetate added/consumed [g]	4188 ± 113/ 3966 ± 131
Ammonia added/consumed [g]	240 ± 4/ 165 ± 7
Y XIS [gCDW/gacetate]	0.27 ± 0.02
μmax [h−1]	0.42 ± 0.00
μoverall feeding phase [h−1]	0.08 ± 0.00
Fluorescence [AU·104]	9537 ± 98
Biomass‐specific fluorescence [AU·104/gCDW·L]	208 ± 1
Relative target protein content [% of soluble protein]	17.7 ± 2.5
eYFP titer [g/L]	2.7 ± 0.3
Y PIX [mgYFP/gCDW]	40.0 ± 1.0
PV overall [g/L·h]	0.10 ± 0.02