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Acute and chronic hypoxia in MCF-7 breast cancer cell line. MCF-7 cells were incubated under normoxia (20% O2) or hypoxia (1% O2) for 1 day (acute) or 5 days (chronic). Hypoxia biomarkers, HIF1α (A) and CA9 (B), were detected by Immunofluorescence and Western blotting, respectively. Ingenuity Pathway Analysis software was used for analysis of RNAseq data to identify major biological themes for acute (C) and chronic (D) hypoxia in MCF-7 cells. Liu et al., unpublished results
