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Generation and characterization of human-induced pluripotent stem cell-endothelial cells (hiPSC-ECs). (A) Schematic representation of the protocol for differentiation of hiPSCs to ECs; (B) Differentiation of hiPSC-EC through a 3D protocol; (C) Flow cytometry assay for the detection of CD31 and CD144 expression post-hiPSC-EC differentiation; (D) Identification of hiPSC-EC by immunofluorescent staining.
