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. 2022 Oct 17;9:922790. doi: 10.3389/fcvm.2022.922790

FIGURE 3.

FIGURE 3

Arterial stenosis of carotid artery is reduced by exposure to human-induced pluripotent stem cell- endothelial cell-extracellular vesicle (hiPSC-EC-EV). (A) Schematic illustration of the carotid artery ligation procedure in the mouse model. (B) Time chart for the in vivo treatment of the vascular tissue with hiPSC-EC-EV and its collection for testing. (C) Analysis of the neointimal hyperplasia formation in the hiPSC-EC-EV-treated vs untreated mice 21 days after surgery by HE staining, and quantification of the intima-to-media ratio in the blood vessels. Scale bar = 100 μm. (D) Representative ultrasound images of the lumen size in the carotid arteries 21 days after the surgery and statistical analysis of the differences. (E) Representative images of ECs immunofluorescently stained for CD31 and PKH67 in the carotid arteries 21 days after the surgery. Data represent mean ± SD from three independent experiments (n = 3). Differences between linked groups were evaluated by a two-tailed Student’s t-test. *p < 0.05 vs. Sham, #p < 0.05 vs. PBS.