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. 2022 Oct 17;9:922790. doi: 10.3389/fcvm.2022.922790

FIGURE 6.

FIGURE 6

Extracellular vesicle (EV)-derived miR-126 promotes human umbilical vein endothelial cell (HUVEC) migration and survival via autophagy regulation. (A) The expression profiles of some common EC miRNAs; (B) The expression levels of miR-126 in hiPSC-EC and hiPSC-EC-EV; (C,D) Light microscopy (bright field) images of the HUVECs in a tube formation (C) and a scratch-wound (D) assay; (E) Ki67 staining (Green) of HUVECs treated with or without miR-126 and 3-MA in the presence of TNF-α; (F) TUNEL (Green) and CD31 (Red) staining of HUVECs treated with or without miR-126 and 3-MA in the presence of TNF-α; (G) Western blotting analysis of apoptosis-specific proteins in the treated HUVECs; (H) Densitometric quantification proteins from the Western blotting image pane in panel (G); Data represent mean ± SD from three independent experiments (n = 3). Differences between linked groups were evaluated by a two-tailed Student’s t-test. *p < 0.05 vs. Con; #p < 0.05 vs. NC; $p < 0.05 vs. miR-126.