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Effect of calorie restriction on serum cytokine TNF-α and IL-6 levels of 6-month-old C57BL/6 mice fed a calorie-restricted diet. Mice were treated as described in the legend to Fig. 8. Serum TNF-α (A) and IL-6 (B) levels were quantitated by ELISA. Each bar represents the mean ± standard error of the mean of five independent measurements. The means for bars labeled with different letters are significantly different (P < 0.05).
