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. 2022 Oct 17;13:1019512. doi: 10.3389/fpls.2022.1019512

Figure 1.

Figure 1

Antipathogen activity and phylogenetic relationship analyses. (A) Comparison of the antipathogen activity of B halotolerans LDFZ001 (LDFZ001) with its relative strains Bacillus subtillis 168 (Bs168) and Bacillus halotolerans F41-3 (BhF41-3) against R. solani Kühn sh-1, a pathogenic fungus strain cased sheath blight disease in crop plants. R. solani Kühn sh-1 inoculated at the center of PDA medium plate was cultured three days at 28°C alone or co-cultured with LDFZ001, Bs168 or BhF41-3, which was inoculated as a scratch line under the R. solani Kühn sh-1 inoculation spot on each plate. (B) Susceptibility analysis of wheat seedlings pretreated with LDFZ001, Bs168 and BhF41-3 to R. solani Kühn Sh-1.The sodium phosphate buffer was used as negative control (control). Wheat seedlings without any treatment were shown in the left as a control, too. (C) Inhibition rates (IRs) of B halotolerans LDFZ001, B subtillis 168 and B halotolerans F41-3 against R. solani Kühn sh-1 in image (A). (D) Disease incidence rates (DIRs) in image (B). Values are the mean ± SD from three independent experiments (n = 30). *P< 0.05. (E) Phylogenetic tree of B halotolerans LDFZ001 and other related taxa was generated based on 16S rRNA sequence. MEGA 7 was used to align the sequences.