Figure 5.
Functional analysis of SFP and MFS transporter genes. (A) Comparison of core genes in the srf gene clusters from B halotolerans LDFZ001, B subtillis 168 and B halotolerans F41-3. (B) A schematic map to show the gRNA sequence positions of SFPe and MFS genes. (C) Δldfz-sfp and Δldfz-mfs generated by respectively editing the SFP and MFS in B halotolerans LDFZ001 led to the loss of antifungal activity. (D) HPLC profile of lipopeptide extracts from WT and mutant Δldfz-sfp and Δldfz-mf.
