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Cycloleucine inhibit myotube formation. (A) qRT-PCR results for MyoD, c-Myc, and Myomarker after treatment with 0, 10, 20, and 30 mM cycloleucine for 24 h. (B) The protein expression of MyHC determined by Western blotting. (C and D) MF20 immunofluorescence staining (C) and myotube area (%) analysis (D) of MF20 immunofluorescence staining after being treated with 0, 10, 20, and 30 mM cycloleucine for 24 h at D2. (*P < 0.05, ***P < 0.001)
