Table 4.
The primers and AT (°C) used for real-time quantitative PCR.
| Name | Sequences (5′ to 3′) | AT | GenBank ID |
|---|---|---|---|
| FAS-QF | AAAGCAGTAAGAAGACCAAC | 57.0 | MH253823.1 |
| FAS-QR | GCTATGACGATTTCCTCCATG | ||
| ACC-QF | ATACGACCCTCGGTGGAT | 59.4 | MH253822.1 |
| ACC-QR | CATAAACGAGCCGAAGTCAA | ||
| SCD-QF | AATACTCCGAGACAGACGCA | 63.3 | KJ818303.1 |
| SCD-QR | GGATCAGGAAGCACATCACTAC | ||
| HSL-QF | CCTGCTAATGGATACCGCT | 59.4 | XM_027172606 |
| HSL-QR | TGCTGGAATGATACCTCTTGT | ||
| CPT1-QF | ATTTGAAGAAGCACCCAGAGTATGT | 64.2 | JN579122.1 |
| CPT1-QR | CCCTTTTATGGACGGAGACAGA | ||
| FABP4-QF | TAGGAAACCGAACCAAGCC | 63.3 | XM_027175558.1 |
| FABP4-QR | CCTCACCGCCACCACAT | ||
| FATP1-QF | TCTGCCGCTACCTGCTGTCT | 65.0 | XM_027162220.1 |
| FATP1-QR | ATCCCACCTTGCCGTCCAT | ||
| LPL-QF | TCATTGTGGTGGACTGGCTT | 63.4 | XM_027159331.1 |
| LPL-QR | CTGTGATCCTGCTGACTTTGTTT | ||
| PPARα-QF | GAATAAGGCTAAAGCACGAGG | 60.3 | MH570217.1 |
| PPARα-QR | CATTTGGGTGACCAGAACAG | ||
| PPARγ-QF | GTCGCTTTCAGAAGTGCCT | 61.3 | XM_027140763.1 |
| PPARγ-QR | GCTTTGGTCAGGGGGAA | ||
| SREBP-1c-QF | CTGGGTCATCGCTTCTTTGTG | 64.5 | KR020013.1 |
| SREBP-1c-QR | TCCTTCGTTGGAGCTTTTGTCT | ||
| AMPK-QF | CTCACCACTTTGCCAAGCCTCAC | 65.0 | KX852428 |
| AMPK-QR | GCCTGTAACTGGGTTCTTCCTCC | ||
| ULK1-QF | GGAGGGTGTTGTGATGTTTGAGGC | 59.0 | XM_027176183.1 |
| ULK1-QR | CTTGCTGGTGTAGGCAGGTTGTG | ||
| Becn1-QF | CTCAACTGGACCGCCTGAAGAAA | 59.0 | XM_027177667.1 |
| Becn1-QR | CACTCCACAGGAACGCTGGGTAAT | ||
| Atg9a-QF | CTGCCCATCCGTTTCCGTTTACC | 59.0 | XM_027160018.1 |
| Atg9a-QR | TTCGGACTTCAGACTCCATTCGTTT | ||
| Atg4b-QF | GCTGCGATGTGGACAGATGATTC | 65.0 | XM_027147236.1 |
| Atg4b-QR | TCTTGTCTATGAAGGCATTGAGGATGT | ||
| Atg5-QF | CAGAACCGTTTTATCTTCTCCTACCG | 59.0 | XM_027142793.1 |
| Atg5-QR | CGTCTACATCTTCAGCTTTCACGACTT | ||
| Atg7-QF | CTCCTATTCAAACCCAGTGCGTCAG | 65.0 | XM_027140729.1 |
| Atg7-QR | ATCAGTGCCTCCAACTGCTCCAC | ||
| LC3B-QF | CCTGACCACGTCAACATGAGCGAACT | 65.0 | XM_027140826.1 |
| LC3B-QR | GGAAATGGCGGCAGACACGGAGA | ||
| SQSTM1-QF | TTTCTCAAACCTCCAAAATGTCGC | 63.3 | KY062772 |
| SQSTM1-QR | GGGAAGTCACGCTTGTGCTCCTT | ||
| 18 S-QF | CCTGAGAAACGGCTACCACATCC | 57.1 | KP938527 |
| 18 S-QR | AGCAACTTTAATATACGCTATTGGAG | ||
| β-actin-QF | CCTAAAGCCAACAGGGAAAA | 59.0 | EU161066 |
| β-actin-QR | ATGGGGCAGAGCATAACC |
AT = annealing temperature; FAS = Fatty acid synthase; ACC = acetyl-CoA carboxylase; SCD = stearoyl-CoA desaturase; HSL = hormone sensitive lipase; CPT1 = carnitine palmitoyl transferase 1; FABP4 = fatty acid binding protein 4; FATP1 = fatty acid transport protein 1; LPL = lipoprotein lipase; PPARα = peroxisome proliferator-activated receptor α; PPARγ = peroxisome proliferator-activated receptor γ; SREBP-1c = sterol regulating element binding protein-1c; AMPK = adenosine 5′-monophosphate-activated protein kinase; ULK1 = uncoordinated 51-like kinase 1; Becn1 = beclin 1; Atg9a = autophagy-related protein 9a; Atg4b = autophagy-related protein 4 b; Atg5 = autophagy-related protein 5; Atg7 = autophagy-related protein 7; LC3B = autophagy marker light chain 3 B; SQSTM1 = sequestosome 1.