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. 2022 Aug 2;11:369–380. doi: 10.1016/j.aninu.2022.07.006

Table 5.

Effect of dietary Ile on muscle FA profiles (% of total FA methyl esters)1.

Item Dietary Ile levels, g/kg
 Pr > F2
5.0 7.5 10.0 12.5 15.0 17.5 20.0 ANOVA Linear Quadratic
SFA 24.82 ± 0.50ab 24.58 ± 0.28a 25.25 ± 0.16ab 25.63 ± 0.74ab 25.72 ± 0.11b 25.11 ± 0.50ab 25.02 ± 0.21ab 0.02 0.21 0.05
C12:0 0.06 ± 0.00 0.07 ± 0.00 0.07 ± 0.01 0.06 ± 0.01 0.07 ± 0.01 0.08 ± 0.00 0.07 ± 0.00 0.40 0.17 0.40
C14:0 1.31 ± 0.05bcd 1.33 ± 0.03cd 1.22 ± 0.11abc 1.14 ± 0.06a 1.16 ± 0.01ab 1.23 ± 0.06abc 1.42 ± 0.06d 0.03 0.73 0.00
C15:0 0.22 ± 0.02 0.19 ± 0.03 0.19 ± 0.02 0.20 ± 0.01 0.20 ± 0.00 0.22 ± 0.02 0.23 ± 0.03 0.17 0.20 0.02
C16:0 17.7 ± 0.58 17.71 ± 0.00 18.06 ± 0.48 18.5 ± 0.58 18.63 ± 0.19 18.23 ± 0.48 18.33 ± 0.39 0.34 0.07 0.21
C17:0 0.44 ± 0.03ab 0.36 ± 0.02a 0.33 ± 0.04a 0.38 ± 0.05a 0.35 ± 0.02a 0.53 ± 0.11b 0.4 ± 0.03ab 0.01 0.30 0.13
C18:0 4.12 ± 0.02a 3.95 ± 0.27a 4.01 ± 0.06a 4.56 ± 0.01c 4.49 ± 0.16bc 4.15 ± 0.15a 4.19 ± 0.04ab 0.02 0.07 0.04
C20:0 0.08 ± 0.01 0.07 ± 0.01 0.04 ± 0 0.06 ± 0.01 0.06 ± 0.02 0.07 ± 0.01 0.07 ± 0.02 0.53 0.60 0.14
C21:0 0.04 ± 0.00 0.04 ± 0.00 0.03 ± 0.00 0.03 ± 0.00 0.03 ± 0.00 0.02 ± 0.00 0.04 ± 0.01 0.49 0.32 0.34
C22:0 0.68 ± 0.06b 0.71 ± 0.10b 1.09 ± 0.24c 0.57 ± 0.14b 0.57 ± 0.15b 0.39 ± 0.05ab 0.11 ± 0.02a 0.00 0.00 0.01
C23:0 0.07 ± 0.01 0.07 ± 0.00 0.13 ± 0.09 0.06 ± 0.00 0.08 ± 0.02 0.12 ± 0.00 0.09 ± 0.07 0.70 0.53 0.89
C24:0 0.04 ± 0.03 0.03 ± 0.02 0.03 ± 0.00 0.02 ± 0.00 0.02 ± 0.00 0.02 ± 0.00 0.02 ± 0.00 0.73 0.13 0.85
MUFA 37.87 ± 1.92ab 37.96 ± 1.08ab 37.74 ± 0.36ab 39.26 ± 1.45b 37.57 ± 0.52ab 37.47 ± 0.16ab 35.82 ± 2.10a 0.04 0.18 0.13
C14:1 0.03 ± 0.00a 0.02 ± 0.00a 0.06 ± 0.00b 0.03 ± 0.00a 0.03 ± 0.00a 0.07 ± 0.01b 0.02 ± 0.00a 0.00 0.17 0.02
C16:1 3.94 ± 0.1 3.83 ± 0.16 4.23 ± 0.00 4.09 ± 0.18 4.05 ± 0.17 4.00 ± 0.07 4.07 ± 0.25 0.37 0.39 0.30
C17:1 0.27 ± 0.02 0.19 ± 0.03 0.19 ± 0.06 0.20 ± 0.07 0.18 ± 0.03 0.24 ± 0.02 0.20 ± 0.00 0.50 0.55 0.21
C18:1n-9 28.97 ± 1.25ab 29.48 ± 1.77ab 29.53 ± 0.43ab 31.71 ± 0.62b 29.73 ± 1.84ab 28.82 ± 0.61ab 27.22 ± 1.45a 0.03 0.22 0.02
C20:1n-9 2.69 ± 0.22 2.68 ± 0.21 2.34 ± 0.51 2.22 ± 0.24 2.15 ± 0.15 2.35 ± 0.26 2.63 ± 0.42 0.47 0.42 0.07
C22:1n-9 0.34 ± 0.07 0.29 ± 0.09 0.34 ± 0.05 0.23 ± 0.03 0.28 ± 0.03 0.45 ± 0.12 0.27 ± 0.22 0.51 0.94 0.71
C24:1n-9 1.59 ± 0.44 1.45 ± 0.27 1.02 ± 0.5 0.75 ± 0.36 1.14 ± 0.91 1.52 ± 0.06 1.39 ± 0.2 0.57 0.85 0.12
PUFA 37.30 ± 1.42ab 37.44 ± 0.79ab 37 ± 0.19ab 35.09 ± 0.71a 36.7 ± 0.64ab 37.4 ± 0.33ab 39.15 ± 2.31b 0.02 0.27 0.03
C18:2n-6 21.53 ± 1.76ab 21.93 ± 0.47b 20.15 ± 0.46ab 20.55 ± 0.17ab 20.47 ± 0.06ab 19.53 ± 0.5a 21.59 ± 0.94ab 0.03 0.21 0.07
C18:3n-3 2.62 ± 0.06 3.17 ± 0.79 2.54 ± 0.57 3.14 ± 0.09 3.52 ± 0.48 2.51 ± 0.56 3.10 ± 0.29 0.36 0.57 0.50
C18:3n-6 1.53 ± 0.01 1.60 ± 0.06 1.82 ± 0.21 1.51 ± 0.27 1.84 ± 0.20 2.21 ± 0.12 2.12 ± 0.95 0.50 0.08 0.65
C20:3n-3 0.37 ± 0.02 0.42 ± 0.02 0.38 ± 0.02 0.36 ± 0.01 0.41 ± 0.08 0.47 ± 0.02 0.48 ± 0.15 0.59 0.13 0.45
C20:3n-6 0.62 ± 0.00 0.65 ± 0.02 0.74 ± 0.08 0.62 ± 0.11 0.81 ± 0.06 0.9 ± 0.05 0.87 ± 0.39 0.46 0.07 0.76
C20:4n-6 0.05 ± 0.01 0.04 ± 0.00 0.04 ± 0.00 0.04 ± 0.00 0.05 ± 0.01 0.08 ± 0.00 0.06 ± 0.04 0.56 0.15 0.67
C20:5n-3 0.57 ± 0.05ab 0.60 ± 0.07ab 0.48 ± 0.11a 0.53 ± 0.05ab 0.65 ± 0.12ab 0.74 ± 0.07b 0.68 ± 0.06ab 0.04 0.05 0.24
C22:6n-3 9.97 ± 0.39 8.99 ± 2.04 10.80 ± 1.68 8.31 ± 0.01 8.91 ± 0.2 10.93 ± 0.56 10.23 ± 2.08 0.40 0.59 0.34
n-3 PUFA 13.55 ± 0.3 13.2 ± 1.18 14.22 ± 0.97 12.35 ± 0.14 13.51 ± 0.48 14.66 ± 1.03 14.49 ± 1.88 0.38 0.23 0.29
n-6 PUFA 23.75 ± 1.73 24.24 ± 0.39 22.78 ± 0.77 22.73 ± 0.56 23.18 ± 0.15 22.73 ± 0.69 24.65 ± 0.43 0.23 0.98 0.04
n-3/n-6 0.57 ± 0.05 0.54 ± 0.05 0.62 ± 0.06 0.54 ± 0.00 0.58 ± 0.01 0.64 ± 0.06 0.58 ± 0.06 0.48 0.35 0.96
EFA 24.16 ± 1.82ab 25.1 ± 1.27b 22.7 ± 1.03ab 23.69 ± 0.26ab 24 ± 0.41ab 22.04 ± 0.06a 24.69 ± 1.24ab 0.02 0.44 0.21

FA = fatty acid; SFA = saturated fatty acid; MUFA = monounsaturated fatty acid; PUFA = polyunsaturated fatty acid; n-3 PUFA = sum of n-3 polyunsaturated fatty acids; n-6 PUFA = sum of n-6 poly-unsaturated fatty acids; EFA = essential fatty acid.

1

The following FA were utilized for calculating the FA classes but they are not listed because blow 0.03% of the total FAME: C4:0, C6:0, C8:0, C10:0, C11:0, C13:0, C15:1, C20:2, and C22:2. Values are means ± SEM (n = 18). Values within the same rows having different superscripts are significantly different (P < 0.05).

2

Significance probability associated with the F-statistic.