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Representative TESA-blots using 15 μg of excreted-secreted antigens from T. cruzi with different biodemes and sera from patients with different forms of chronic Chagas' disease: cardiac form (A), asymptomatic form (B), digestive form (C), and mixed form (D). Numbers 1, 2, and 3 designate sera from different patients; number 4 designates normal serum. Lanes: Y, Y strain; W, WSL strain; S, 12SF strain; C, Colombiana strain; M, molecular mass markers (205, 116, 97.4, 66, 45, and 29 kDa).
