Table 1.

Brain tissue staining: reagent, results, and corresponding cells.

Staining reagent	Results and corresponding cells
Immunohistochemistry (IHC) staining with anti-Ki67, and anti-DCX antibodies	DAPI+/Ki67+ cells ​= ​cells in mitotic phase DCX ​+ ​cells ​= ​immature neurons DAPI+/Ki67+/DCX ​+ ​cells ​= ​mitotic immature neurons
IHC staining with anti-Nestin, anti-DCX, and anti-GFAP antibodies	Nestin+/DCX- cells ​= ​neural progenitor cells Nestin+/DCX ​+ ​cells ​= ​proliferating neuroblasts Nestin-/DCX ​+ ​cells ​= ​immature neurons Nestin+/GFAP ​+ ​cells ​= ​neural stem cell Nestin-/GFAP ​+ ​cells ​= ​mature astrocytes GFAP ​+ ​cells ​= ​astrocytes
IHC staining with anti-BrdU, and anti-NeuN antibodies	BrdU ​+ ​cells ​= ​newly generated cells NeuN ​+ ​cells ​= ​neuronal cell bodies NeuN+/BrdU ​+ ​cells ​= ​new mature neurons
IHC staining with anti-BrdU, and anti-S100ß/S100 antibodies	BrdU+/S100ß+ cells ​= ​new astrocytes S100+ cells ​= ​astrocytes
IHC staining with anti-Iba1 or anti-CD11b antibodies	Iba1+ or CD11b ​+ ​cells ​= ​activated microglia
IHC staining with anti-cFos antibody	cFos ​+ ​cells ​= ​cells with neuronal activation
Cresyl Violet	Nissl substance in neurons