TABLE 2.
Interassay variabilitya
| CIRID control sample | Concn (CV) ofb:
|
|||||
|---|---|---|---|---|---|---|
| β2M (mg/liter) (n = 22) | NPT (nmol/liter) (n = 25) | sIL-2R (U/ml) (n = 14)c | sTNF-RII (ng/ml) (n = 30) | TNF-α (pg/ml) (n = 10) | IFN-γ (U/liter) (n = 5) | |
| Normal | 0.79 ± 0.07 (8.8) | 4.02 ± 0.58 (14.4) | 463 ± 49 (10.5) | 1.62 ± 0.14 (8.6) | 10.0 ± 1.6 (16.0) | <50 (NA)d |
| Elevated | 2.57 ± 0.16 (6.2) | 17.6 ± 0.71 (4.3) | 1690 ± 131 (7.7) | 4.72 ± 0.23 (4.3) | 32.3 ± 2.3 (7.2) | 515 ± 35 (5.3) |
a
All replicates were obtained from the Center for Interdisciplinary Research in Immunology and Disease (CIRID), as described in Materials and Methods.
b
Values are means ± standard deviations; CVs are percentages. NPT, neopterin.
c
Different CIRID control samples were used to test intra-assay and interassay variability for sIL-2R.
d
<50, less than lower limit of detection. NA, not applicable.