TABLE 1.
Primers and peptides used in diagnostic studies
| Primer or peptide | Sequence (5′→3′) | Target organism | Nucleotide positionb | Amino acid positionc | Product size (bp) |
|---|---|---|---|---|---|
| Primers | |||||
| FTSZ-F-CONa | GCTTGGCGCAGCAGTGACA | Bartonella | 189–208 | 1,580 | |
| FTSZ-R-CON | GGCGACGTAAAAATGCTGGTATTTCC | Bartonella | 1768–1743 | ||
| BB-F | AAATCAGACAGCTTCTCATCC | B. bacilliformis | 1043–1063 | 612 | |
| BB-R | GAATCCTGAGAAGACACTTTG | B. bacilliformis | 1655–1634 | ||
| BH-F | GCCGCAAAGTTCTTTTCATG | B. henselae | 1044–1063 | 354 | |
| BH-R | AGGTGAACGCGCTTGTATTTG | B. henselae | 1398–1378 | ||
| BQ-F | GTCTCAGACTTCTTTTCATC | B. quintana | 1044–1063 | 367 | |
| BQ-R | GAGCCCGCATCTGTTGCATTTCCT | B. quintana | 1411–1388 | ||
| Peptides | |||||
| BB-1 | NKNVQKVSSQDSSVYAPHRSTKLQSRAL | B. bacilliformis | 541–568 | ||
| BH-3 | SSAHGQISNAPRVQVSRSSQS | B. henselae | 409–429 | ||
| BQ-5 | NSVSYGSNVVHGQISNATRPQFSRCSQQSMAAS | B. quintana | 404–436 |
a
FTSZ-F-CON and FTSZ-R-CON are derived from conserved Bartonella ftsZ sequences.
b
Position within the ftsZBb ORF (23) for conserved primers and within the ORF of the ftsZ homolog of the target organism for species-specific primers.
c
Position within the FtsZ homolog of the target organism.