Figure 1.
(A) Schematic illustration of the pMPC-functionalized liposomes, depicting pMPC polymer conjugated to phosphatidylethanolamine (DSPE) lipid present at the liposome surface at 5% (mol/mol).18 The pMPCylated liposomes are composed of HSPC with 40% (mol/mol) cholesterol and 5% (mol/mol) SA. Liposomes were loaded with antimicrobial agents: either SMX on their own or together with EA. (B) Representative cryo-TEM images of unloaded pMPCylated LUVs after 1 h incubation in (a) 10 mM and (b) 40 mM Ca(Ac)2 solution and (c) PEG-LUVs in 40 mM solution of Ca(Ac)2. White arrows indicate adhesion points, black arrow indicates no adhesion. Scale bar, 50 nm. (C) Confocal microscopy images of time-lapse acquisition of pMPC-GUVs stained with DiI dye (red), interacting upon addition of 40 mM Ca(Ac)2. Scale bar, 20 μm.